Difference between revisions of "Part:BBa K1179069"
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Encodes the sequence for the CAG promoter.
 
Encodes the sequence for the CAG promoter.
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[http://2017.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt] tried to improve this part at [https://parts.igem.org/Part:BBa_K2217013# BBa_K2217013] by making it RFC 10 compatible. CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:11, 23 October 2017

CAG promoter

This part is submitted under the RFC 65 standard.

Encodes the sequence for the CAG promoter.

[http://2017.igem.org/Team:AFCM-Egypt# Team:AFCM-Egypt] tried to improve this part at BBa_K2217013 by making it RFC 10 compatible. CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    COMPATIBLE WITH RFC[1000]