Difference between revisions of "Part:BBa K2235004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The sequence has been optimized for E.coli strain, K12 using the codon optimization tool in IDT website. | |
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===Source=== | ===Source=== | ||
| − | Obtained as a part of a G-block and it was PCR amplified and | + | Obtained as a part of a G-block and it was PCR amplified and cloned into the plasmid pSB1C3. |
===References=== | ===References=== | ||
| + | 1. Kleanthous C, Walker D. Immunity proteins: enzyme inhibitors that avoid the active site. Trends in Biochemical Sciences. 2001;26(10):624-631. | ||
Latest revision as of 12:15, 23 October 2017
Colicin E2 immunity protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence has been optimized for E.coli strain, K12 using the codon optimization tool in IDT website.
Source
Obtained as a part of a G-block and it was PCR amplified and cloned into the plasmid pSB1C3.
References
1. Kleanthous C, Walker D. Immunity proteins: enzyme inhibitors that avoid the active site. Trends in Biochemical Sciences. 2001;26(10):624-631.