Difference between revisions of "Part:BBa K2235004"
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This part encodes the immunity protein 2 which binds and inhibits the nicking DNase Colicin E2. | This part encodes the immunity protein 2 which binds and inhibits the nicking DNase Colicin E2. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Colicin E2 is a non-specific DNase, found in E.coli. It randomly cleaves the chromosome overwhelming the DNA repair machinery and leads to cytotoxicity in the host and its extracellular environment. The expression of Immunity protein 2 (Im2) binds to the neutral site of Colicin and neutralizes its action. | Colicin E2 is a non-specific DNase, found in E.coli. It randomly cleaves the chromosome overwhelming the DNA repair machinery and leads to cytotoxicity in the host and its extracellular environment. The expression of Immunity protein 2 (Im2) binds to the neutral site of Colicin and neutralizes its action. | ||
The interplay between these two enzymes can be employed in a suicide system attempting to ensure biocontainment of genetic material. A combination of two or more robust gene switches could regulate the expression of these two and the main priority is to minimize any leaky expression of the toxin. | The interplay between these two enzymes can be employed in a suicide system attempting to ensure biocontainment of genetic material. A combination of two or more robust gene switches could regulate the expression of these two and the main priority is to minimize any leaky expression of the toxin. | ||
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<partinfo>BBa_K2235004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2235004 SequenceAndFeatures</partinfo> | ||
| + | ===References=== | ||
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| + | 1. Kleanthous C, Walker D. Immunity proteins: enzyme inhibitors that avoid the active site. Trends in Biochemical Sciences. 2001;26(10):624-631. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Latest revision as of 12:14, 23 October 2017
Colicin E2 immunity protein
This part encodes the immunity protein 2 which binds and inhibits the nicking DNase Colicin E2.
Usage and Biology
Colicin E2 is a non-specific DNase, found in E.coli. It randomly cleaves the chromosome overwhelming the DNA repair machinery and leads to cytotoxicity in the host and its extracellular environment. The expression of Immunity protein 2 (Im2) binds to the neutral site of Colicin and neutralizes its action.
The interplay between these two enzymes can be employed in a suicide system attempting to ensure biocontainment of genetic material. A combination of two or more robust gene switches could regulate the expression of these two and the main priority is to minimize any leaky expression of the toxin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Kleanthous C, Walker D. Immunity proteins: enzyme inhibitors that avoid the active site. Trends in Biochemical Sciences. 2001;26(10):624-631.