Difference between revisions of "Part:BBa K2235004:Design"

 
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===Design Notes===
 
===Design Notes===
Codon optimization for E.coli
+
The sequence has been optimized for E.coli strain, K12 using the codon optimization tool in IDT website.
  
  

Revision as of 11:48, 23 October 2017


Colicin E2 immunity protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for E.coli strain, K12 using the codon optimization tool in IDT website.


Source

Obtained as a part of a G-block and it was PCR amplified and incorporated into the plasmid

References