Difference between revisions of "Part:BBa K2505004"
Line 24: Line 24:
 
==Characterization==
 
==Characterization==
  
−
===Sub part===
+
===Materials & Methods===
−
 
+
−
<!-- Add more about the biology of this part here
+
−
 
+
−
 
+
−
<!-- -->
+
−
<span class='h3bb'>Sequence and Features</span>
+
−
<partinfo>BBa_K2505003 SequenceAndFeatures</partinfo>
+
−
 
+
−
<!-- Uncomment this to enable Functional Parameter display
+
−
===Functional Parameters===
+
−
<partinfo>BBa_K2505003 parameters</partinfo>
+
−
<!-- -->
+

Revision as of 07:49, 23 October 2017

atipt4-IVS-IRES-log1-polyA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1193
    Illegal XhoI site found at 2243
    Illegal XhoI site found at 2255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 881


This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. atipt4 gene and log1 gene is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.

Characterization

===Materials & Methods===