Difference between revisions of "Part:BBa K2505004"
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<span style="margin-left: 25px;">This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. <i>atipt4</i> gene and <i>log1</i> gene is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase '''[EC:2.5.1.112]''': cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.
 
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===Characterization===
 
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===Usage and Biology===
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<span style="margin-left: 25px;">This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. <i>atipt4</i> gene and <i>log1</i> gene is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase '''[EC:2.5.1.112]''': cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.
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===Characterization===

Revision as of 07:38, 23 October 2017

atipt4-IVS-IRES-log1-polyA Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1193
    Illegal XhoI site found at 2243
    Illegal XhoI site found at 2255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 881


This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP(isopentenyl adenine: kind of cytokinin) as a signaling molecule. atipt4 gene and log1 gene is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.


===Characterization===