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| − | __NOTOC__
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| − | <h1>Introduction</h1>
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| − | <partinfo>BBa_K2368017 short</partinfo>
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| − | <h3>General</h3>
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| − | <p>Sst2 protein is an important negative regulatory factor of the pheromone GPSTP. In order to improve the detection sensitivity, we design this part to knock out the sst2 gene.</p>
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| − | <h3>Our experiment</h3>
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| − | <p>Similarly, we design 3 pairs of primers and the marker is Histone synthesis gene, short termed His. We get 3 fragments by PCR and they have the overlap areas with each other. Then, the complete fragment observed by OE-PCR is converted to the yeast. Additionally, the colony is chosen on the relevant nutritional deficiency medium, so that only the positive cloning can survival on it.</p>
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| − | <!-- Add more about the biology of this part here
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| − | ===Usage and Biology===
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| − | <h2>Sequence and Features</h2>
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| − | <partinfo>BBa_K2368017 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K2368017 parameters</partinfo>
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