Difference between revisions of "Part:BBa K2332004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | We had to reverse translate and codon optimize the protein sequence for its usage in E. coli and ensure no illegal sites were present. | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005. | ||
Latest revision as of 00:20, 23 October 2017
EL222, photosensitive DNA-binding protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 81
Illegal AgeI site found at 306 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to reverse translate and codon optimize the protein sequence for its usage in E. coli and ensure no illegal sites were present.
Source
Sequence obtained from the reverse translation of EL222 (UniProtKB/Swiss-Prot: [http://www.uniprot.org/uniprot/Q2NB98 Q2NB98.1]) then codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
References
Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.