Difference between revisions of "Part:BBa K2332011:Design"
Paola handal (Talk | contribs) (→Design Notes) |
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===Source=== | ===Source=== | ||
− | + | Intimin protein sequence obtained from UniProtKB: [http://www.uniprot.org/uniprot/P43261#sequences P43261], reverse translated and codon optimised for E. coli. | |
− | Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences | + | Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001. |
− | + | SpyTag was obtained from: [https://parts.igem.org/Part:BBa_K1159201 BBa_K1159201]. The DNA sequence was synthesised by Integrated DNA Technologies (IDT) | |
===References=== | ===References=== |
Revision as of 23:58, 22 October 2017
Blue light inducible expression of Intimin'-SpyTag (Pblind Intimin'-SpyTag)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 880
Illegal NgoMIV site found at 1621 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We decided to fuse SpyTag to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins (Wentzel et al., 2001). We also included a linker between Intimin and SpyTag to facilitate mobility of SpyTag on the cell surface.
Source
Intimin protein sequence obtained from UniProtKB: [http://www.uniprot.org/uniprot/P43261#sequences P43261], reverse translated and codon optimised for E. coli. Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001. SpyTag was obtained from: BBa_K1159201. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)