Difference between revisions of "Part:BBa K2332020:Design"

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===Design Notes===
 
===Design Notes===
 +
Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: [https://www.ncbi.nlm.nih.gov/nuccore/5726577 AF170104.1], from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli.
  
 
===Source===
 
===Source===
−
Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: [https://www.ncbi.nlm.nih.gov/nuccore/5726577 AF170104.1], from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
+
Vibrio fischeri Genbank: [https://www.ncbi.nlm.nih.gov/nuccore/5726577 AF170104.1]codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
  
 
===References===
 
===References===
 +
Chong G, Kimyon Ö, Manefield M. Quorum Sensing Signal Synthesis May Represent a Selective Advantage Independent of Its Role in Regulation of Bioluminescence in Vibrio fischeri. PLoS ONE. 2013;8(6):e67443.

Latest revision as of 23:19, 22 October 2017


LuxCDABE (E. coli bioluminescence)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
    Illegal AgeI site found at 2829
    Illegal AgeI site found at 3357
    Illegal AgeI site found at 3938
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 5263
    Illegal AgeI site found at 5478
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: AF170104.1, from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli.

Source

Vibrio fischeri Genbank: AF170104.1codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References

Chong G, Kimyon Ö, Manefield M. Quorum Sensing Signal Synthesis May Represent a Selective Advantage Independent of Its Role in Regulation of Bioluminescence in Vibrio fischeri. PLoS ONE. 2013;8(6):e67443.