Difference between revisions of "Part:BBa K2332030"
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This construct displays GFP fused with SpyTag on the cell surface of E. coli cells. Intimin' (159kDa) is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display. | This construct displays GFP fused with SpyTag on the cell surface of E. coli cells. Intimin' (159kDa) is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display. | ||
− | It can be also used in combination with cells expressing our Intimin'-SpyCatcher construct variants. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are protein binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. | + | It can be also used in combination with cells expressing our Intimin'-SpyCatcher construct variants to analyse their co-localization. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are protein binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. |
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Revision as of 22:06, 22 October 2017
Intimin'-GFP-SpyTag
This construct displays GFP fused with SpyTag on the cell surface of E. coli cells. Intimin' (159kDa) is a truncated version of Intimin (EaeA intimin) which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display. It can be also used in combination with cells expressing our Intimin'-SpyCatcher construct variants to analyse their co-localization. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) are protein binding partners that originate from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal NgoMIV site found at 1542 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2636