Difference between revisions of "Part:BBa K2332017:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | We removed the stop codong in GFP and placed the SpyTag sequence | ||
To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli. | To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli. | ||
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===Source=== | ===Source=== |
Revision as of 21:52, 22 October 2017
GFP-SpyTag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Design Notes
We removed the stop codong in GFP and placed the SpyTag sequence To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.
Source
https://parts.igem.org/Part:BBa_K1159201 was the source of the SpyTag sequence https://parts.igem.org/Part:BBa_E0040 was for the GFP