Difference between revisions of "Part:BBa K124002"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K124002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K124002 SequenceAndFeatures</partinfo>
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Group: Tel-Hai 2017
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Author: (the authors of the improvement)
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Summary: (a 1-3 sentence summary of your improvement)
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the documentation of your improvement (text, graphs, pictures, tables, etc.)
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Include sections for purpose, results, methods, etc. (if applicable)
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Uploads: (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.) Note: All files pertaining to a contribution must be uploaded to the Registry.
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Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast.
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This is a strong constitutive yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase. Also called TDH3 or GAPDH.
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Studies have found the promoter activity of  TDH3 decreased significantly when glycerol or xylose was supplied as the carbon source and at high temperature (42 °C) . Oxygen conditions had non-significant effect.
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About the protein - the promoter was taken from the Glyceraldehyde-3-phosphate dehydrogenase peptide (GAPDH), that involves in glycolysis and gluconeogenesis. The protein is a tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate, detected in the cytoplasm and cell wall. GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria.
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http://onlinelibrary.wiley.com/doi/10.1002/yea.1806/full#bib17
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https://www.ncbi.nlm.nih.gov/pubmed/26164057
  
  

Revision as of 21:14, 22 October 2017

Yeast GPD (TDH3) Promoter

Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Group: Tel-Hai 2017 Author: (the authors of the improvement) Summary: (a 1-3 sentence summary of your improvement) the documentation of your improvement (text, graphs, pictures, tables, etc.) Include sections for purpose, results, methods, etc. (if applicable) Uploads: (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.) Note: All files pertaining to a contribution must be uploaded to the Registry.

Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast.


This is a strong constitutive yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase. Also called TDH3 or GAPDH. Studies have found the promoter activity of TDH3 decreased significantly when glycerol or xylose was supplied as the carbon source and at high temperature (42 °C) . Oxygen conditions had non-significant effect.

About the protein - the promoter was taken from the Glyceraldehyde-3-phosphate dehydrogenase peptide (GAPDH), that involves in glycolysis and gluconeogenesis. The protein is a tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate, detected in the cytoplasm and cell wall. GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria.


http://onlinelibrary.wiley.com/doi/10.1002/yea.1806/full#bib17

https://www.ncbi.nlm.nih.gov/pubmed/26164057