Difference between revisions of "Part:BBa K2350004:Design"

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===Design Notes===
 
===Design Notes===
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<h6>Primer</h6>
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<h4>Primer</h4>
  
 
<ol>
 
<ol>

Revision as of 17:12, 22 October 2017


This part produces a part of nitrate channel protein from Synechocystis sp. PCC 6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1179
    Illegal BamHI site found at 549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer

  1. P0001 　　5'aattcgcggccgcttctagagttgacggctagctcagtcctaggtattgtgctagctactagagaaagaggagaaa 3'
  2. P0002 　　5'agtatttctcctctttctctagtagctagcacaatacctaggactgagctagccgtcaactctagaagcggccgcg 3'
  3. P0003 　　5'tactagatgagtaatttttcccgaag 3'
  4. P0004 　　5'agatgagtaatttttcccgaag 3'
  5. P0005 　　5'ttaagtctattaaagcttgaccaggcatcaaataaaacga 3'
  6. P0006 　　5'tcgttttatttgatgcctggtcaagctttaatagacttaa 3'
  7. P0007 　　5'tgcagcggccgctactagtatataaacgcagaaaggccca 3'
  8. P0008 　　5'gcggccgctactagtatataaacgcagaaaggccca 3'

Source

This part produces a part of nitrate channel protein from Synechocystis sp. PCC 6803 .

References

  • Koropatkin NM1, Pakrasi HB, Smith TJ. Atomic structure of a nitrate-binding protein crucial for photosynthetic productivity. 2006 Jun 27;103(26):9820-5. Epub 2006 Jun 15.
  • Ye Wang, Wenbin Li, Yaeesh Siddiqi, James R. Kinghorn, Shiela E. Unkles and Anthony D. M. Glass. Evidence for post-translational regulation of NrtA, the Aspergillus nidulans high-affinity nitrate transporter. DOI: 10.1111/j.1469-8137.2007.02135.x • Source: PubMed
  • Alexova R, Haynes PA, Ferrari BC, Neilan BA. Comparative protein expression in different strains of the bloom-forming cyanobacterium Microcystis aeruginosa. 2011 Sep;10(9):M110.003749. doi: 10.1074/mcp.M110.003749. Epub 2011 May 24.
  • Omata T, Ohmori M, Arai N, Ogawa T. Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate transport. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6612-6.
  • Naureen Akhtar, Eugenia Karabika, James R. Kinghorn, Anthony D.M. Glass, Shiela E. Unkles, and Duncan A. Rouch. High-affinity nitrate/nitrite transporters NrtA and NrtB of Aspergillus nidulans exhibit high specificity and different inhibitor sensitivity. Microbiology. 2015 Jul; 161(Pt 7): 1435–1446. doi: 10.1099/mic.0.000088
  • James R. Kinghorn, Joan Sloan, Ghassan J. M. Kana'n, Edisio R. DaSilva, Duncan A. Rouch, and Shiela E. Unkles. Missense Mutations That Inactivate the Aspergillus nidulans nrtA Gene Encoding a High-Affinity Nitrate Transporter. Genetics. 2005 Mar; 169(3): 1369–1377. doi: 10.1534/genetics.104.036590
  • Maeda S, Omata T. Substrate-binding lipoprotein of the cyanobacterium Synechococcus sp. strain PCC 7942 involved in the transport of nitrate and nitrite. J Biol Chem. 1997 Jan 31;272(5):3036-41.