Difference between revisions of "Part:BBa K2371000:Design"

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The first two Biobricks are the two split T7 polymerase sequence we adopted. We provide them for other team to leverage them through other methods. We choose the split site suggested by the team of Tiyun Han, Quan Chen and Haiyan Liu.( Tiyun,H.et al.,2016.Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase.ACS Synthetic Biology,http://pubs.acs.org on October 31, 2016.)
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This biobrick is expected to generate the protein N-T7-dCas9 synthesized by part of split T7 polymerase and dCas9. The sequence of NT7 and dCas9 are linked via a linker. In consideration of the protein’s activity influenced by its function, the His-Tag must be linked to the C terminus of dCas9.

Revision as of 11:26, 22 October 2017

This biobrick is expected to generate the protein N-T7-dCas9 synthesized by part of split T7 polymerase and dCas9. The sequence of NT7 and dCas9 are linked via a linker. In consideration of the protein’s activity influenced by its function, the His-Tag must be linked to the C terminus of dCas9.