Difference between revisions of "Part:BBa K2232011"

Revision as of 09:47, 22 October 2017

RBS_C125-TupA_rrnBT1

Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125, TupA is a cytoplasmic protein consisting of 489 amino acid residues with a molecular mass of 57.3kDa. TupA homologues are distributed widely among strains of alkaliphilic Bacillus possessing TUP. The function of the TupA protein is not clear, although it was demonstrated that TupA is involved in TUP synthesis. One of the biological functions of TUP, is its involvement in maintaining pH homeostasis at a high pH conditions, under which there is a low level of the antiporter activity. In the cell walls of C-125, a high density of fixed anion charges is generated by dissociation of carboxyl groups of the predominant acidic polymer(the PGlu moiety of TUP), function as an obstacle repulsing hydroxyl ions abundant in the milieu and as a reservoir capturing hydrogen ions generated intracellularly by aerobic respiration.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 995
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 815
Illegal BsaI.rc site found at 1207

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2232011 short</partinfo>
-promoter + coding protein
+Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125, TupA is a cytoplasmic protein consisting of 489 amino acid residues with a molecular mass of 57.3kDa. TupA homologues are distributed widely among strains of alkaliphilic Bacillus possessing TUP. The function of the TupA protein is not clear, although it was demonstrated that TupA is involved in TUP synthesis. One of the biological functions of TUP, is its involvement in maintaining pH homeostasis at a high pH conditions, under which there is a low level of the antiporter activity.
+In the cell walls of C-125, a high density of fixed anion charges is generated by dissociation of carboxyl groups of the predominant acidic polymer(the PGlu moiety of TUP), function as an obstacle repulsing hydroxyl ions abundant in the milieu and as a reservoir capturing hydrogen ions generated intracellularly by aerobic respiration.
 <!-- Add more about the biology of this part here