Difference between revisions of "Part:BBa I15008"
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===New usage=== | ===New usage=== | ||
A novel far-red fluorescent protein evolved from APCα from Trichodesmium erythraeum, called smURFP, can covalently attaches a biliverdin (BV) chromophore without a lyase, unlike its precursor APC which should use an auxiliary protein known as a lyase | A novel far-red fluorescent protein evolved from APCα from Trichodesmium erythraeum, called smURFP, can covalently attaches a biliverdin (BV) chromophore without a lyase, unlike its precursor APC which should use an auxiliary protein known as a lyase | ||
| − | to incorporate phycocyanobilin. In addition, phycocyanobilin (PCB) is synthesized from BV, and PCB do not exists in mammals but BV does. So BV is | + | to incorporate phycocyanobilin. In addition, phycocyanobilin (PCB) is synthesized from BV, and PCB do not exists in mammals but BV does. So BV is better as a chromophore in some way, along with smURFP. |
| + | |||
| + | In our projext, we use HO-1 gene for two task: one is to produce BV in E.coli BL21, the other is to be a element in co-expression system (with fluorescent protein smURFP). | ||
===New infomation=== | ===New infomation=== | ||
Revision as of 08:14, 22 October 2017
heme oxygenase (ho1) from Synechocystis
One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
Usage and Biology
ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis. PCB associates with Cph8, creating a light responsive protein complex. Functions in tandem with BBa_I15009 for PCB biosynthesis. PCB then associates with Part:BBa_I15010, a light responsive Cph8/EnvZ fusion protein.
Additional Supplements
The infomation below is updated by TJU_China of iGEM 2017.
New usage
A novel far-red fluorescent protein evolved from APCα from Trichodesmium erythraeum, called smURFP, can covalently attaches a biliverdin (BV) chromophore without a lyase, unlike its precursor APC which should use an auxiliary protein known as a lyase to incorporate phycocyanobilin. In addition, phycocyanobilin (PCB) is synthesized from BV, and PCB do not exists in mammals but BV does. So BV is better as a chromophore in some way, along with smURFP.
In our projext, we use HO-1 gene for two task: one is to produce BV in E.coli BL21, the other is to be a element in co-expression system (with fluorescent protein smURFP).
New infomation
The original part has a barcode after coding sequence. So we submit a new part Part:BBa_K2328062 (without the barcode) for our composite parts. But we will upload all the data and infomation in both pages.
In addition, in order to make this part express better in hosts of our project (several intestinal bacteria), we optimize the codons of the part and submit another two new parts: Part:BBa_K2328003 for E.coli, EHEC, Citrobacter rodentium, Lactococcus Iactis, Bacaeroides fragilis, Enterococcus faecalis and Clostridium difficile and Part:BBa_K2328004 for Bifidobacterium longum. We do codon optimization for two obligate anaerobes just want to comfirm that this gene cannot work at all in obligate anarobes, which means HO-1 gene doesn't make any sense in these two bacteria, at least for now.
Results
We use this part to prduce Biliverdin (BV) in E.coli BL21. For the subsequent experiments, we use our codon-optimized gene to express HO-1 in facultative anarobes.
Reference
[1]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]