Difference between revisions of "Part:BBa I15008"

(→‎Additional Supplements)
Line 9: Line 9:
 
ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis.  PCB associates with Cph8, creating a light responsive protein complex.  Functions in tandem with BBa_I15009 for PCB biosynthesis.  PCB then associates with [[Part:BBa_I15010]], a light responsive Cph8/EnvZ fusion protein.
 
ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis.  PCB associates with Cph8, creating a light responsive protein complex.  Functions in tandem with BBa_I15009 for PCB biosynthesis.  PCB then associates with [[Part:BBa_I15010]], a light responsive Cph8/EnvZ fusion protein.
  
−
==Additional Supplements==
 
−
The infomation below is updated by TJU_China of iGEM 2017.
 
  
−
==New usage==
 
−
 
−
 
−
==New infomation==
 
−
The original part has a barcode after coding sequence. So we submit a new part [[Part:BBa_K2328062]] (without the barcode) for our composite parts. But we will upload all the data and infomation in both pages.
 
−
 
−
In addition, in order to make this part express better in hosts of our project (several intestinal bacteria), we optimize the codons of the part and submit another two new parts: [[Part:BBa_K2328003]] for E.coli, EHEC, Citrobacter rodentium, Lactococcus Iactis, Bacaeroides fragilis, Enterococcus faecalis and Clostridium difficile and [[Part:BBa_K2328004]] for Bifidobacterium longum. We do codon optimization for two obligate anaerobes just want to comfirm that this gene cannot work at all in obligate anarobes, which means HO-1 gene doesn't make any sense in these two bacteria, at least for now.
 
−
 
−
==Results==
 
−
We use this part to prduce Biliverdin (BV) in E.coli BL21. For the subsequent experiments, we use our codon-optimized gene to express HO-1 in facultative anarobes.
 
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 07:58, 22 October 2017

heme oxygenase (ho1) from Synechocystis

One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.


Usage and Biology

ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis. PCB associates with Cph8, creating a light responsive protein complex. Functions in tandem with BBa_I15009 for PCB biosynthesis. PCB then associates with Part:BBa_I15010, a light responsive Cph8/EnvZ fusion protein.


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]