Difference between revisions of "Part:BBa I15008"
Jiangzhongyi (Talk | contribs) (→Additional Supplements) |
Jiangzhongyi (Talk | contribs) (→Additional Supplements) |
||
| Line 12: | Line 12: | ||
The infomation below is updated by TJU_China of iGEM 2017. | The infomation below is updated by TJU_China of iGEM 2017. | ||
| + | ==New usage== | ||
| − | + | ==New infomation== | |
The original part has a barcode after coding sequence. So we submit a new part [[Part:BBa_K2328062]] (without the barcode) for our composite parts. But we will upload all the data and infomation in both pages. | The original part has a barcode after coding sequence. So we submit a new part [[Part:BBa_K2328062]] (without the barcode) for our composite parts. But we will upload all the data and infomation in both pages. | ||
In addition, in order to make this part express better in hosts of our project (several intestinal bacteria), we optimize the codons of the part and submit another two new parts: [[Part:BBa_K2328003]] for E.coli, EHEC, Citrobacter rodentium, Lactococcus Iactis, Bacaeroides fragilis, Enterococcus faecalis and Clostridium difficile and [[Part:BBa_K2328004]] for Bifidobacterium longum. We do codon optimization for two obligate anaerobes just want to comfirm that this gene cannot work at all in obligate anarobes, which means HO-1 gene doesn't make any sense in these two bacteria, at least for now. | In addition, in order to make this part express better in hosts of our project (several intestinal bacteria), we optimize the codons of the part and submit another two new parts: [[Part:BBa_K2328003]] for E.coli, EHEC, Citrobacter rodentium, Lactococcus Iactis, Bacaeroides fragilis, Enterococcus faecalis and Clostridium difficile and [[Part:BBa_K2328004]] for Bifidobacterium longum. We do codon optimization for two obligate anaerobes just want to comfirm that this gene cannot work at all in obligate anarobes, which means HO-1 gene doesn't make any sense in these two bacteria, at least for now. | ||
| + | ==Results== | ||
We use this part to prduce Biliverdin (BV) in E.coli BL21. For the subsequent experiments, we use our codon-optimized gene to express HO-1 in facultative anarobes. | We use this part to prduce Biliverdin (BV) in E.coli BL21. For the subsequent experiments, we use our codon-optimized gene to express HO-1 in facultative anarobes. | ||
Revision as of 07:57, 22 October 2017
heme oxygenase (ho1) from Synechocystis
One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
Usage and Biology
ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis. PCB associates with Cph8, creating a light responsive protein complex. Functions in tandem with BBa_I15009 for PCB biosynthesis. PCB then associates with Part:BBa_I15010, a light responsive Cph8/EnvZ fusion protein.
Additional Supplements
The infomation below is updated by TJU_China of iGEM 2017.
New usage
New infomation
The original part has a barcode after coding sequence. So we submit a new part Part:BBa_K2328062 (without the barcode) for our composite parts. But we will upload all the data and infomation in both pages.
In addition, in order to make this part express better in hosts of our project (several intestinal bacteria), we optimize the codons of the part and submit another two new parts: Part:BBa_K2328003 for E.coli, EHEC, Citrobacter rodentium, Lactococcus Iactis, Bacaeroides fragilis, Enterococcus faecalis and Clostridium difficile and Part:BBa_K2328004 for Bifidobacterium longum. We do codon optimization for two obligate anaerobes just want to comfirm that this gene cannot work at all in obligate anarobes, which means HO-1 gene doesn't make any sense in these two bacteria, at least for now.
Results
We use this part to prduce Biliverdin (BV) in E.coli BL21. For the subsequent experiments, we use our codon-optimized gene to express HO-1 in facultative anarobes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]