Difference between revisions of "Part:BBa K2350020:Design"
 
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===Design Notes===
 
===Design Notes===

Latest revision as of 07:46, 22 October 2017


R0010-B0034-Holin-B0010-B0012-J23106-B0034-Endolysin-B0010-B0012


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1035
    Illegal NheI site found at 1058
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1362
    Illegal AgeI site found at 1432
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

　　In our 2017 NYMU project, the endolysin and holin both are from enterobacteria phage T4, for the reason that we don’t know whether endolysin and holin from different species could cooperate well.

Source

　　The endolysin and holin we used are from iGEM released parts, BBa_K112806 and BBa_K112000, respectively. They originated from the previous team, 2008 UC Berkeley.

References

  • 2008 UC Berkeley Team: http://2008.igem.org/Team:UC_Berkeley
  • 2014 Peking Team: http://2014.igem.org/Team:Peking/Suicide