Difference between revisions of "Part:BBa I15008"

Revision as of 07:41, 22 October 2017

heme oxygenase (ho1) from Synechocystis

One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.

Usage and Biology

ho1 oxidizes the heme group using a ferredoxin cofactor, generating biliverdin IXalpha and representing the first of two steps in phycocyanobilin (PCB) biosynthesis. PCB associates with Cph8, creating a light responsive protein complex. Functions in tandem with BBa_I15009 for PCB biosynthesis. PCB then associates with Part:BBa_I15010, a light responsive Cph8/EnvZ fusion protein.

Additional Supplements

The data below is updated by TJU_China of iGEM 2017.

The original part has a barcode after coding sequence. So we submit a new part Part:BBa_K2328062 (without the barcode). But we will upload all the data and infomation in both pages. In addition, in order to make this part express better in hosts of our project (several intestinal bacteria), we optimize the codons of the part and submit another two new parts: Part:BBa_K2328003 for E.cooli, EHEC, Citrobacter rodentium, Lactococcus Iactis, Bacaeroides fragilis, Enterococcus faecalis and Clostridium difficile and Part:BBa_K2328004 for Bifidobacterium longum.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 12: / Line 12: @@
 The data below is updated by TJU_China of iGEM 2017.
-The original part has a barcode after coding sequence. So we submit a new part [[Part:BBa_K2328062]] (without the barcode). But we will upload all the data and infomation in both pages.
+The original part has a barcode after coding sequence. So we submit a new part [[Part:BBa_K2328062]] (without the barcode). But we will upload all the data and infomation in both pages. In addition, in order to make this part express better in hosts of our project (several intestinal bacteria), we optimize the codons of the part and submit another two new parts: [[Part:BBa_K2328003]] for E.cooli, EHEC, Citrobacter rodentium, Lactococcus Iactis, Bacaeroides fragilis, Enterococcus faecalis and Clostridium difficile and [[Part:BBa_K2328004]] for Bifidobacterium longum.
 <span class='h3bb'>Sequence and Features</span>