Difference between revisions of "Part:BBa K2505004"
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<partinfo>BBa_K2505004 short</partinfo> | <partinfo>BBa_K2505004 short</partinfo> | ||
| − | This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP as a signaling molecule. | + | <span style="margin-left: 25px;">This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP as a signaling molecule. atipt4 gene and log1 gene is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP(isopentenyl adenine: kind of cytokinin). LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor. |
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| + | ===Characterization=== | ||
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Revision as of 07:33, 22 October 2017
atipt4-IVS-IRES-log1-polyA
This gene codes complete iP synthesis enzymes. This gene was introduced to human cell, EA.hy926 cell and produces iP as a signaling molecule. atipt4 gene and log1 gene is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase [EC:2.5.1.112]: cytokinin synthase) that necessary for a synthesis of iP(isopentenyl adenine: kind of cytokinin). LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for maturation of iP precursor.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1193
Illegal XhoI site found at 2243
Illegal XhoI site found at 2255 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 739
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 881