Difference between revisions of "Part:BBa K2505003"
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<partinfo>BBa_K2505003 short</partinfo> | <partinfo>BBa_K2505003 short</partinfo> | ||
− | <span style="margin-left: 25px;"> | + | <span style="margin-left: 25px;">log1 gene is derived from <i>A.thaliana</i>, optimized for <i>H.sapiens</i> codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor. |
===Characterization=== | ===Characterization=== | ||
− | <span style="margin-left: 25px;">We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP | + | <span style="margin-left: 25px;">We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 07:31, 22 October 2017
IVS-IRES-log1
log1 gene is derived from A.thaliana, optimized for H.sapiens codon in reference to the codon usage. LOG1 is an enzyme(putative lysine decarboxylase family) that necessary for a maturation of iP precursor.
Characterization
We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP precursor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 225
Illegal XhoI site found at 1275
Illegal XhoI site found at 1287 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]