Difference between revisions of "Part:BBa K2505003"
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===Characterization===
 
===Characterization===
  
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<span style="margin-left: 25px;">We tested the expression of LOG1 protein in EA.hy626 cell that necessary for maturation of iP(isopentenyl adenine: kind of cytokinin).  
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<span style="margin-left: 25px;">We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP(isopentenyl adenine: kind of cytokinin).  
  
 
  <!-- Add more about the biology of this part here
 
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Revision as of 06:39, 22 October 2017


IVS-IRES-log1

Log1 gene is derived from A.thaliana and optimized for H.sapiens codon in reference to codon usage. LOG1 is a enzyme(putative lysine decarboxylase family) that necessary for maturation of iP(isopentenyl adenine: kind of cytokinin).


Characterization

We tested the expression of LOG1 protein in human cell(EA.hy626) that necessary for maturation of iP(isopentenyl adenine: kind of cytokinin).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
    Illegal XhoI site found at 1275
    Illegal XhoI site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]