Difference between revisions of "Part:BBa K2407102:Design"

 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
This sequence is modified for use in yeast or other AT-rich fungi.
 
This sequence is modified for use in yeast or other AT-rich fungi.
 +
This part expresses the vika gene which will react with vox site in the mating so as to trigger Vika-Vox system.
  
  

Revision as of 15:52, 21 October 2017


control the expression of vika gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast or other AT-rich fungi. This part expresses the vika gene which will react with vox site in the mating so as to trigger Vika-Vox system.


Source

We use overlap PCR to connect the segments(TDH3p,vika and TDH2t).

References