Difference between revisions of "Part:BBa K2407100:Design"
(→‎Design Notes)
Line 9: Line 9:
 
This sequence is modified for use in yeast or other AT-rich fungi.
 
This sequence is modified for use in yeast or other AT-rich fungi.
 
yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742
 
yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742
−
It is a composite part to control the expression of HO gene. What's more, trigger the transformation of their mating type.
+
It is a composite part to control the expression of HO gene. What's more, it triggers the transformation of their mating type.
  
 
===Source===
 
===Source===

Revision as of 15:46, 21 October 2017


a composite part to control the expression of HO gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
    Illegal BglII site found at 902
    Illegal BglII site found at 1185
    Illegal BglII site found at 1869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast or other AT-rich fungi. yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742 It is a composite part to control the expression of HO gene. What's more, it triggers the transformation of their mating type.

Source

We connect the segments(Gal-HO-CYC1) by T4 DNA Ligase.


References