Difference between revisions of "Part:BBa K2450101"

Revision as of 15:44, 21 October 2017

TEV protease tagged with mCherry

A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.

Usage and Biology

TEV protease is a well-characterised specific protease from the Tobacco Etch Virus, with a cleavage sequence of Glu-Asn-Leu-Tyr-Phe-Gln-(CUT)-Gly.

This is a non-self-cleaving protease as we wanted a constant level of TEV protease produced.

We have used TEV protease as both a substitute for cruzipain, another specific protease, and as a signal amplifier after simulated cruzipain cleavage.

This part does not require any other parts to be functional.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1444
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+TEV protease is a well-characterised specific protease from the Tobacco Etch Virus, with a cleavage sequence of Glu-Asn-Leu-Tyr-Phe-Gln-(CUT)-Gly.
+This is a non-self-cleaving protease as we wanted a constant level of TEV protease produced.
+We have used TEV protease as both a substitute for cruzipain, another specific protease, and as a signal amplifier after simulated cruzipain cleavage.
+This part does not require any other parts to be functional.
 <!-- -->