Difference between revisions of "Part:BBa K2332040:Design"
Paola handal (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
− | Removal of ATG from GFP fusion protein | + | Removal of ATG from GFP for a more efficient expression of this fusion protein |
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===Source=== | ===Source=== |
Revision as of 15:16, 21 October 2017
Blue light inducible expression of Intimin'-GFP-SpyTag (Pblind Intimin'-GFP-SpyTag)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2844
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2844
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2844
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2844
Illegal NgoMIV site found at 880
Illegal NgoMIV site found at 1621 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2715
Design Notes
Removal of ATG from GFP for a more efficient expression of this fusion protein
Source
Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag: BBa_K1159201 GFPmut 3b: Part:BBa_E0040