Difference between revisions of "Part:BBa K2450201:Design"
Line 13: Line 13:
  
 
TetR from BBa_K106669
 
TetR from BBa_K106669
−
TEV cleavage sequence from Merck
+
<p>TEV cleavage sequence from Merck</p>
−
CFP from AddGene
+
<p>CFP from AddGene</p>
  
 
===References===
 
===References===

Revision as of 14:40, 21 October 2017


TetR with TEV cleavage site and CFP tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.

Source

TetR from BBa_K106669

TEV cleavage sequence from Merck

CFP from AddGene

References