Difference between revisions of "Part:BBa K2450201:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | |||
We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains. | We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains. | ||
− | |||
− | |||
===Source=== | ===Source=== | ||
− | BBa_K106669 | + | TetR from BBa_K106669 |
TEV cleavage sequence from Merck | TEV cleavage sequence from Merck | ||
CFP from AddGene | CFP from AddGene | ||
===References=== | ===References=== |
Revision as of 14:37, 21 October 2017
TetR with TEV cleavage site and CFP tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.
Source
TetR from BBa_K106669 TEV cleavage sequence from Merck CFP from AddGene