Difference between revisions of "Part:BBa K2334004"
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| − | YgfU is a high-capacity transporter for uric acid in Escherichia coli, which is homologous to nucleobase transporters of the ubiquitous family NCS2. In our project, it's used as the urate transporter in E. coli. | + | YgfU is a high-capacity transporter for uric acid in Escherichia coli, which is homologous to nucleobase transporters of the ubiquitous family NCS2. In our project, it's used as the urate transporter in E. coli, whose expression is driven by J23100. RiboJ is used for the quantitive pathway construction. |
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Revision as of 03:42, 21 October 2017
J23100 + RiboJ + B0034 + YgfU, Urate Transporter Generator
YgfU is a high-capacity transporter for uric acid in Escherichia coli, which is homologous to nucleobase transporters of the ubiquitous family NCS2. In our project, it's used as the urate transporter in E. coli, whose expression is driven by J23100. RiboJ is used for the quantitive pathway construction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 397
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 579
- 1000COMPATIBLE WITH RFC[1000]