Difference between revisions of "Part:BBa K2300001"
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− | The Macquarie Australia iGEM team have successfully transformed E. coli (DH5α) with a hydrogenase gene cluster capable of converting glucose into hydrogen gas. This was achieved with our main bio-brick submission, the Hydrogen Gas Producing Gene Cluster. | + | The Macquarie Australia iGEM team have successfully transformed <i>E. coli</i> (DH5α) with a hydrogenase gene cluster capable of converting glucose into hydrogen gas. This was achieved with our main bio-brick submission, the Hydrogen Gas Producing Gene Cluster. |
− | This gene cluster translates to a complex consists of the [FeFe] hydrogenase enzyme (Hyd1) from Chlamydomonas reinhardtii (Mulder et al., 2011), ferredoxin, ferredoxin-NADPH-reductase (FNR) and the maturation enzymes (HydEF and HydG). Together these enzymes work cohesively to produce our desired hydrogen gas product whilst avoiding the detrimental emission present in current hydrogen gas production processes. | + | This gene cluster translates to a complex consists of the [FeFe] hydrogenase enzyme (Hyd1) from <i>Chlamydomonas reinhardtii</i> (Mulder et al., 2011), ferredoxin, ferredoxin-NADPH-reductase (FNR) and the maturation enzymes (HydEF and HydG). Together these enzymes work cohesively to produce our desired hydrogen gas product whilst avoiding the detrimental emission present in current hydrogen gas production processes. |
These bacteria could represent an alternate, clean, renewable source of hydrogen for electrical power generation. We hope that the progress we have made will lead to further exploration of the Hydrogen Gas Producing Gene Cluster’s potential. | These bacteria could represent an alternate, clean, renewable source of hydrogen for electrical power generation. We hope that the progress we have made will lead to further exploration of the Hydrogen Gas Producing Gene Cluster’s potential. | ||
Revision as of 03:38, 21 October 2017
Hydrogen Gas Producing Gene Cluster
The final composite part to create hydrogen
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1792
Illegal NheI site found at 5180
Illegal NheI site found at 5390
Illegal NheI site found at 5834
Illegal NotI site found at 3584 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4232
Illegal NgoMIV site found at 8188
Illegal AgeI site found at 5147
Illegal AgeI site found at 6790 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 6015
Illegal BsaI.rc site found at 3635
Illegal BsaI.rc site found at 3747
Biology & Literature
The Macquarie Australia iGEM team have successfully transformed E. coli (DH5α) with a hydrogenase gene cluster capable of converting glucose into hydrogen gas. This was achieved with our main bio-brick submission, the Hydrogen Gas Producing Gene Cluster. This gene cluster translates to a complex consists of the [FeFe] hydrogenase enzyme (Hyd1) from Chlamydomonas reinhardtii (Mulder et al., 2011), ferredoxin, ferredoxin-NADPH-reductase (FNR) and the maturation enzymes (HydEF and HydG). Together these enzymes work cohesively to produce our desired hydrogen gas product whilst avoiding the detrimental emission present in current hydrogen gas production processes. These bacteria could represent an alternate, clean, renewable source of hydrogen for electrical power generation. We hope that the progress we have made will lead to further exploration of the Hydrogen Gas Producing Gene Cluster’s potential.
Part Verification
Discuss Clark electrode and Octopus here.
Protein information
References
MULDER, D. W., SHEPARD, E. M., MEUSER, J. E., JOSHI, N., KING, P. W., POSEWITZ, M. C., BRODERICK, J. B. & PETERS, J. W. 2011. Insights into [FeFe]-hydrogenase structure, mechanism, and maturation. Structure, 19, 1038-1052.