Difference between revisions of "Part:BBa K2300001"
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===Biology & Literature===
 
===Biology & Literature===
  
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Background here such as what is in Intro
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The Macquarie Australia iGEM team have successfully transformed E. coli (DH5α) with a hydrogenase gene cluster capable of converting glucose into hydrogen gas. This was achieved with our main bio-brick submission, the Hydrogen Gas Producing Gene Cluster.
 +
This gene cluster translates to a complex consists of the [FeFe] hydrogenase enzyme (Hyd1) from Chlamydomonas reinhardtii (Mulder et al., 2011), ferredoxin, ferredoxin-NADPH-reductase (FNR) and the maturation enzymes (HydEF and HydG). Together these enzymes work cohesively to produce our desired hydrogen gas product whilst avoiding the detrimental emission present in current hydrogen gas production processes.
 +
These bacteria could represent an alternate, clean, renewable source of hydrogen for electrical power generation. We hope that the progress we have made will lead to further exploration of the Hydrogen Gas Producing Gene Cluster’s potential.
  
 
===Part Verification===
 
===Part Verification===

Revision as of 03:25, 21 October 2017


Hydrogen Gas Producing Gene Cluster

The final composite part to create hydrogen

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1792
    Illegal NheI site found at 5180
    Illegal NheI site found at 5390
    Illegal NheI site found at 5834
    Illegal NotI site found at 3584
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4232
    Illegal NgoMIV site found at 8188
    Illegal AgeI site found at 5147
    Illegal AgeI site found at 6790
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6015
    Illegal BsaI.rc site found at 3635
    Illegal BsaI.rc site found at 3747

Biology & Literature

The Macquarie Australia iGEM team have successfully transformed E. coli (DH5α) with a hydrogenase gene cluster capable of converting glucose into hydrogen gas. This was achieved with our main bio-brick submission, the Hydrogen Gas Producing Gene Cluster. This gene cluster translates to a complex consists of the [FeFe] hydrogenase enzyme (Hyd1) from Chlamydomonas reinhardtii (Mulder et al., 2011), ferredoxin, ferredoxin-NADPH-reductase (FNR) and the maturation enzymes (HydEF and HydG). Together these enzymes work cohesively to produce our desired hydrogen gas product whilst avoiding the detrimental emission present in current hydrogen gas production processes. These bacteria could represent an alternate, clean, renewable source of hydrogen for electrical power generation. We hope that the progress we have made will lead to further exploration of the Hydrogen Gas Producing Gene Cluster’s potential.

Part Verification

Discuss Clark electrode and Octopus here.

Protein information

References