Difference between revisions of "Part:BBa K2374002:Design"

 
(→‎Design Notes)
Line 9: Line 9:
 
site direct mutangenesis:
 
site direct mutangenesis:
 
EcoR I (325):GAATTC->GAGTTC
 
EcoR I (325):GAATTC->GAGTTC
−
 
+
There are other mutations during the parts construction by accident:
−
 
+
1142: A->G; aa: Lys->Arg
 +
1154: A->G; aa: Glu->Gly
  
 
===Source===
 
===Source===

Revision as of 22:34, 20 October 2017


GAL80ts (temperature dependent)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 993
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
    Illegal BglII site found at 615
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 27
    Illegal BsaI site found at 73


Design Notes

site direct mutangenesis: EcoR I (325):GAATTC->GAGTTC There are other mutations during the parts construction by accident: 1142: A->G; aa: Lys->Arg 1154: A->G; aa: Glu->Gly

Source

Chromosome: XIII; NC_001145.3 (171594..172901) NOTE: Gal80ts is a mutation based on the gene above.


References