Difference between revisions of "Part:BBa K2325002:Design"

 
 
Line 12: Line 12:
  
 
===Source===
 
===Source===
−
 
+
The Formamidase (FOR) gene is cloned from the genome of Paenibacillus pasadenensis. This sequence have been accepted as a patent owned by Mr. Wenyong Lou.
 +
The Phosphite dehydrogenase (PTDH) is amplified from the genome of Klebsiella pneumonia.
  
  
  
 
===References===
 
===References===

Latest revision as of 20:10, 20 October 2017


Formamidase & Phosphite dehydrogenase fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 69
    Illegal NgoMIV site found at 556
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutagenesis to remove internal PstI site.


Source

The Formamidase (FOR) gene is cloned from the genome of Paenibacillus pasadenensis. This sequence have been accepted as a patent owned by Mr. Wenyong Lou. The Phosphite dehydrogenase (PTDH) is amplified from the genome of Klebsiella pneumonia.


References