Difference between revisions of "Part:BBa K2387003:Experience"
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This construct is used to determine the functionality of the araC/pBAD promoter. Furthermore it can be used as a positive control in many fluorescence based experiments. | This construct is used to determine the functionality of the araC/pBAD promoter. Furthermore it can be used as a positive control in many fluorescence based experiments. | ||
| − | eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls | + | eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls. |
| − | Used | + | |
| + | |||
| + | Used protocol can be found at http://2017.igem.org/Team:Wageningen_UR/Notebook/Protocols | ||
<div class="figure-center-imagebox"> | <div class="figure-center-imagebox"> | ||
Revision as of 07:48, 20 October 2017
This construct is used to determine the functionality of the araC/pBAD promoter. Furthermore it can be used as a positive control in many fluorescence based experiments.
eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls.
Used protocol can be found at http://2017.igem.org/Team:Wageningen_UR/Notebook/Protocols
"
We clearly show that increasing the concentration of L-arabinose leads to a higher fluorescent signal due to increased protein production.
Applications of BBa_K2387003
User Reviews
UNIQ0e4db3684aa1ea6e-partinfo-00000000-QINU UNIQ0e4db3684aa1ea6e-partinfo-00000001-QINU