Difference between revisions of "Part:BBa K2250007"
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| − | This is | + | This is the secretion part of warrior 2 in our project. LuxI make the warrior 2 able to secrete the 3OC6HSL to the medium and then enter the other cell and kill warrior 1/beggar 1/farmer 1. This can be realized by 3OC6HSL binding to LuxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1/farmer 1 unable to survive under the Chloramphenicol. Also, the GFP is used to distinguish between group 1 (containing warrior 1/beggar 1/farmer 1) and group 2 (containing warrior 2/beggar 2/farmer 2). GFP represents group 2. |
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Latest revision as of 04:34, 20 October 2017
Pconst-LuxI-Pconst-GFP
This is the secretion part of warrior 2 in our project. LuxI make the warrior 2 able to secrete the 3OC6HSL to the medium and then enter the other cell and kill warrior 1/beggar 1/farmer 1. This can be realized by 3OC6HSL binding to LuxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1/farmer 1 unable to survive under the Chloramphenicol. Also, the GFP is used to distinguish between group 1 (containing warrior 1/beggar 1/farmer 1) and group 2 (containing warrior 2/beggar 2/farmer 2). GFP represents group 2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 856
Illegal NheI site found at 879 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 698
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1554