Difference between revisions of "Part:BBa K2250005"
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| − | This is the reception part of warrior 2/beggar 2/farmer 2 in our project. This part | + | This is the reception part of warrior 2/beggar 2/farmer 2 in our project. This part makes warrior 2/beggar 2/farmer 2 able to recept the C4HSL secreted by warrior 1 so that the warrior 1 can kill warrior 2/beggar 2/farmer 2. This can be realized by C4HSL binding to RhlR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 2/beggar 2/farmer 2 unable to survive under the Chloramphenicol. |
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Revision as of 04:30, 20 October 2017
Pconst-RhlR-Prhl-LacI-PlacI-CmR
This is the reception part of warrior 2/beggar 2/farmer 2 in our project. This part makes warrior 2/beggar 2/farmer 2 able to recept the C4HSL secreted by warrior 1 so that the warrior 1 can kill warrior 2/beggar 2/farmer 2. This can be realized by C4HSL binding to RhlR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 2/beggar 2/farmer 2 unable to survive under the Chloramphenicol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2159
Illegal BamHI site found at 301 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776