Difference between revisions of "Part:BBa K2230009"
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| + | PI promoter [BBa_K861170] is a modified version to Pcar with correction of -10 position in the promoter region, which gives the promoter stronger activity and a weaker CRP interaction. The PI promoter [BBa_K861170] was used as a major part in the work of team NCKU_Tainan in 2016. These two parts have been used in our work. | ||
| + | PhlF repressor system contains the repressor PhlF [BBa_K1725041] and the PhlF repressible promoter [BBa_K1725001] created by Glasgow in 2015. PhlF could repress GFP fluorescence intensity by 83-fold according to the study of Glasgow’s work. | ||
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| + | Cloning: PI-RBS-PhlF-T was amplified from RBS + PhlF repressor + terminator (BBa_K1725041) using a primer with PI-RBS (B0032) sequence. The PCR product was ligated to pSB1C3 cut by XbaI and PstI. | ||
| + | Function: Repressor PhlF can be dose-dependently expressed in an increasing concentrations of glucose. According to teams WHU-China 2012 & NCKU_Tainan 2016, the promoter, PI, is glucose responsive. | ||
Revision as of 04:15, 20 October 2017
PI promoter [BBa_K861170] is a modified version to Pcar with correction of -10 position in the promoter region, which gives the promoter stronger activity and a weaker CRP interaction. The PI promoter [BBa_K861170] was used as a major part in the work of team NCKU_Tainan in 2016. These two parts have been used in our work.
PhlF repressor system contains the repressor PhlF [BBa_K1725041] and the PhlF repressible promoter [BBa_K1725001] created by Glasgow in 2015. PhlF could repress GFP fluorescence intensity by 83-fold according to the study of Glasgow’s work.
Cloning: PI-RBS-PhlF-T was amplified from RBS + PhlF repressor + terminator (BBa_K1725041) using a primer with PI-RBS (B0032) sequence. The PCR product was ligated to pSB1C3 cut by XbaI and PstI.
Function: Repressor PhlF can be dose-dependently expressed in an increasing concentrations of glucose. According to teams WHU-China 2012 & NCKU_Tainan 2016, the promoter, PI, is glucose responsive.