Difference between revisions of "Part:BBa K2206010"

Revision as of 23:40, 19 October 2017

Toehold switch for hsa-miR-27b-3p (second series)

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch

Bba_X and hsa-miR-15b-5p contain roughly half of the trigger sequence each. Colocalization of the split trigger RNA sequences results in a functional trigger RNA that is capable of activating the toehold switch. This part codes for a toehold switch that contains a region that is complementary to the complete trigger RNA sequence formed of Bba_X and hsa-miR-15b-5p Therefore, this part can be used to regulate the expression of any protein in response to presence of both Bba_X and hsa-miR-15b-5p.

We found that the transcripts produced by our other toehold switch parts were toxic to E.coli. We therefore recommend using this part with a non-leaky promoter. For reference, we found the part BBa_K808000 to be too leaky.

This part contains a strong RBS sequence.

Sequence and Features