Difference between revisions of "Part:BBa K2255002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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A RBS is present before the start codon allowing the operon.
 
  
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This Thioestherase<ref>Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).</ref> is necessary to the production of 2-cis-decenenoic acid by ''Pseudomonas aeruginosa'', so we search its DNA sequence in the genome to create our first biobrick. This enzyme gene is on a operon along the other gene crucial for the formation of 2-cis-decenenoic acid.
  
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We took the gene coding for the right enzyme and optimized its sequence for a production in ''E.coli''. Then, a ribosome binding site (RBS) was added to the sequence in order to make an operon with [https://parts.igem.org/Part:BBa_K2255000 BBa_K2255000] and [https://parts.igem.org/Part:BBa_K2255001 BBa_K2255001]. Finaly, a [https://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] prefix and sufix was added.
  
 
===Source===
 
===Source===

Revision as of 15:11, 19 October 2017


RBS + Thioesterase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Thioestherase[1] is necessary to the production of 2-cis-decenenoic acid by Pseudomonas aeruginosa, so we search its DNA sequence in the genome to create our first biobrick. This enzyme gene is on a operon along the other gene crucial for the formation of 2-cis-decenenoic acid.

We took the gene coding for the right enzyme and optimized its sequence for a production in E.coli. Then, a ribosome binding site (RBS) was added to the sequence in order to make an operon with BBa_K2255000 and BBa_K2255001. Finaly, a Rfc10 prefix and sufix was added.

Source

This sequence came from Pseudomonas aeruginosa.

References

  1. ↑ Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).