Difference between revisions of "Part:BBa K2206001"

Revision as of 13:56, 19 October 2017

Toehold switch for hsa-miR-27b-3p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

This part codes for a toehold switch that contains a region that is complementary to the microRNA hsa-miR-27b-3p (the trigger RNA). In the presence of hsa-miR-27b-3p, translation of the downstream protein coding region occurs.

This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-27b-3p present in a system.

The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found BBa_K808000 was too leaky).

This part contains a strong RBS sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K2206001 short</partinfo>
-{{Template:CLSB-UK 17 TS|microRNA=hsa-miR-27b-3p|reporter=GFPmut3b}}
+Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
+This part codes for a toehold switch that contains a region that is complementary to the microRNA hsa-miR-27b-3p (the trigger RNA). In the presence of hsa-miR-27b-3p, translation of the downstream protein coding region occurs.
+This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-27b-3p present in a system.
+The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found BBa_K808000 was too leaky).
+This part contains a strong RBS sequence.
 <!-- Add more about the biology of this part here