Difference between revisions of "Part:BBa K2250005"
Shuailin10 (Talk | contribs) |
Shuailin10 (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2250005 short</partinfo> | <partinfo>BBa_K2250005 short</partinfo> | ||
| − | This is | + | This is the reception part of warrior 2/beggar 2/farmer 2 in our project. This part make warrior 2/beggar 2/farmer 2 able to recept the C4HSL secreted by warrior 1 so that the warrior 1 can kill warrior 1/beggar 1/farmer 1. This can be realized by C4HSL binding to RhlR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 2/beggar 2/farmer 2 unable to survive under the Chloramphenicol. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:11, 19 October 2017
Pconst-RhlR-Prhl-LacI-PlacI-CmR
This is the reception part of warrior 2/beggar 2/farmer 2 in our project. This part make warrior 2/beggar 2/farmer 2 able to recept the C4HSL secreted by warrior 1 so that the warrior 1 can kill warrior 1/beggar 1/farmer 1. This can be realized by C4HSL binding to RhlR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 2/beggar 2/farmer 2 unable to survive under the Chloramphenicol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2159
Illegal BamHI site found at 301 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776