Difference between revisions of "Part:BBa K2250004"
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<partinfo>BBa_K2250004 short</partinfo> | <partinfo>BBa_K2250004 short</partinfo> | ||
| − | This is correspond to the reception part of warrior 1/beggar 1/farmer 1 in our project. This part make the | + | This is correspond to the reception part of warrior 1/beggar 1/farmer 1 in our project. This part make the warrior 1/beggar 1/farmer 1 able to recept the 3OC6HSL secreted by warrior 2 so that the warrior 2 can kill warrior 1/beggar 1/farmer 1. This can be realized by 3OC6HSL binding to LuxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1/farmer 1 unable to survive under the Chloramphenicol. |
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Revision as of 07:56, 19 October 2017
Pconst-luxR-Plux-LacI-PlacI-CmR
This is correspond to the reception part of warrior 1/beggar 1/farmer 1 in our project. This part make the warrior 1/beggar 1/farmer 1 able to recept the 3OC6HSL secreted by warrior 2 so that the warrior 2 can kill warrior 1/beggar 1/farmer 1. This can be realized by 3OC6HSL binding to LuxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1/farmer 1 unable to survive under the Chloramphenicol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2215
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985