Difference between revisions of "Part:BBa K2250004"

Revision as of 07:42, 19 October 2017

Pconst-luxR-Plux-LacI-PlacI-CmR

This is correspond to the reception part of warrior 1/ beggar 1/ farmer 1 in our project. This part make the E.coli MG1666(ΔlacI) able to recept the 3OC6HSL secreted by soldier 2 so that the warrior 2 can kill warrior 1/beggar 1/ farmer. This can be realized by 3OC6HSL binding to luxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1 unable to survive under the Chloramphenicol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2215
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 985

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2250004 short</partinfo>
-This is correspond to the reception part of warrior 1/ beggar 1/ in our project. This part make the E.coli MG1666(&#916;lacI) able to recept the 3OC6HSL secreted by soldier 2 so that the warrior 2 can kill warrior 1/beggar 1. This can be realized by 3OC6HSL binding to luxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1 unable to survive under the Chloramphenicol.
+This is correspond to the reception part of warrior 1/ beggar 1/ farmer 1 in our project. This part make the E.coli MG1666(&#916;lacI) able to recept the 3OC6HSL secreted by soldier 2 so that the warrior 2 can kill warrior 1/beggar 1/ farmer. This can be realized by 3OC6HSL binding to luxR, activating the expression of LacI, and then LacI inhibiting the expression of CmR, making the warrior 1/beggar 1 unable to survive under the Chloramphenicol.
 <!-- Add more about the biology of this part here