Difference between revisions of "Part:BBa K2404002"
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 21:26, 18 October 2017


PDF1.2 shortened - a jasmonic acid-induced promoter

The PDF1.2 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to respond to jasmonic acid.

It is a truncated version of BBa_K1467103

This promotor includes 500bp upstream of the PDF1.2 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the pSB1C3 plasmid using BsmBI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 517