Difference between revisions of "Part:BBa K2443010"

 
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<partinfo>BBa_K2443010 short</partinfo>
 
<partinfo>BBa_K2443010 short</partinfo>
  
isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
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isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
  
  

Revision as of 14:47, 18 October 2017


IleRS optimized for expression in E. coli

isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1419
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147
    Illegal SapI.rc site found at 1538