Difference between revisions of "Part:BBa I751000"

 
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<partinfo>BBa_I751000 short</partinfo>
 
<partinfo>BBa_I751000 short</partinfo>
  
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pBR322 has Pst I site in its Amp resistant gene, so you can't introduce Biobrick site(Pst I included) in this plasmid.
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pBR322 has Pst I site in its Amp resistant gene. It prevents us from introducing Biobrick site(Pst I included) to this plasmid.
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For that reason, we introduced a silent mutation at the site. The mutation is A546C(DNA sequence) in the Amp resistant gene. Introducing this mutation allowed us to aim at constructing pBR standard plasmid.
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For that reason, we introduced a silent mutation at the site, in order not to restrict Amp resistant gene. The mutation is A546C(DNA sequence) in the Amp resistant gene. Introducing this mutation allowed us to aim at constructing pBR standard plasmid.
  
 
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<!-- Add more about the biology of this part here

Revision as of 16:05, 19 October 2007

pBR322 (Pst I site mutated)

pBR322 has Pst I site in its Amp resistant gene. It prevents us from introducing Biobrick site(Pst I included) to this plasmid. For that reason, we introduced a silent mutation at the site, in order not to restrict Amp resistant gene. The mutation is A546C(DNA sequence) in the Amp resistant gene. Introducing this mutation allowed us to aim at constructing pBR standard plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3433
    Illegal SapI site found at 2350