Difference between revisions of "Part:BBa K2387030:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | A long flexible linker was used to fuse CpxR and eYFPc to maximize the availability of | + | A long flexible linker was used to fuse CpxR and eYFPc to maximize the availability of eYFPc[155-238] and thus increase the chances to reassemble with eYFPn[1-154]. |
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===Source=== | ===Source=== | ||
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[https://parts.igem.org/Part:BBa_K2387006 eYFPc[155-238]] was created in during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project]. | [https://parts.igem.org/Part:BBa_K2387006 eYFPc[155-238]] was created in during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project]. | ||
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===References=== | ===References=== |
Latest revision as of 12:40, 16 October 2017
CpxR-eYFPc fusion + araC/pBAD promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 1283 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1694 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
A long flexible linker was used to fuse CpxR and eYFPc to maximize the availability of eYFPc[155-238] and thus increase the chances to reassemble with eYFPn[1-154].
Source
eYFP originates from the jellyfish Aequorea victoria
araC/pBAD is an endogenous E. coli promoter
These original parts are taken from the iGEM repository; [http://parts.igem.com/BBa_E0030 eYFP] [http://parts.igem.com/BBa_I0030 araC/pBAD promoter] [http://parts.igem.com/BBa_B0034 RBS BBa_B0034] [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] [http://parts.igem.com/Part:BBa_K2387002 CpxR]
eYFPc[155-238] was created in during [http://2017.igem.org/Team:Wageningen_UR Wageningen_URs 2017 iGEM project].