Difference between revisions of "Part:BBa K2387030"
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CpxR and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS. | CpxR and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS. | ||
| − | It can be used in combination with the fusion [http://parts.igem.com/Part:BBa_K2387011 CpxR-eYFPc[155-238]] to visualize the activation of the Cpx pathway via Bimolecular Fluorescence Complementation | + | It can be used in combination with the fusion [http://parts.igem.com/Part:BBa_K2387011 CpxR-eYFPc[155-238]] to visualize the activation of the Cpx pathway via Bimolecular Fluorescence Complementation by using the CpxA-CpxR interaction. |
| + | Furthermore, it is used as a negative control in visualizing the Cpx pathway by combining BiFC to CpxR dimerization [http://parts.igem.com/Part:BBa_K2387032 CpxR-eYFPn(1-154)-CpxR-eYFPc(155-238)] | ||
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Latest revision as of 12:37, 16 October 2017
CpxR-eYFPc fusion + araC/pBAD promoter
The [http://parts.igem.com/Part:BBa_K2387006 C-terminus of eYFP] is fused to Cpx response regulator [http://parts.igem.com/Part:BBa_K2387002 CpxR] in order to visualize the activation of the Cpx pathway. The fusion is put under the control of the L-arabinose inducible araC/pBAD promoter. Strong RBS BBa_B0034 is used to regulate transcription.
CpxR and eYFPn were linked together using a [http://parts.igem.com/Part:BBa_K1486004 Flexible Linker] consisting of two times the amino acids GGGGS.
It can be used in combination with the fusion [http://parts.igem.com/Part:BBa_K2387011 CpxR-eYFPc[155-238]] to visualize the activation of the Cpx pathway via Bimolecular Fluorescence Complementation by using the CpxA-CpxR interaction. Furthermore, it is used as a negative control in visualizing the Cpx pathway by combining BiFC to CpxR dimerization [http://parts.igem.com/Part:BBa_K2387032 CpxR-eYFPn(1-154)-CpxR-eYFPc(155-238)]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 1283 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1694 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961